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Alomone Labs
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Boster Bio
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Image Search Results
Journal: British Journal of Pharmacology
Article Title: Ca 2+ paradox injury mediated through TRPC channels in mouse ventricular myocytes
doi: 10.1111/j.1476-5381.2010.00986.x
Figure Lengend Snippet: Functional expression of transient receptor potential canonical (TRPC) channels in mouse ventricular myocytes. (A–C) Activation of TRPC current by thapsigargin recorded under conditions where Na+, Ca2+ and K+ channel currents were minimized. (A) Time course of changes in membrane current measured at +50 and −110 mV during the voltage-ramp protocol (from +50 to −110 mV), before and during exposure to thapsigargin (Thap, 2 µmol·L−1), without and then with 2-APB (20 µmol·L−1). (B) I-V relationships measured at time points (a, b, c) indicated in (A). (C) Difference currents obtained by digital subtraction as indicated (b-a: thapsigargin-activated current; b-c: 2-APB-sensitive current). (D) Immunostaining of TRPC1, TRPC3 and TRPC4. Scale bar in all panels, 25 µm.
Article Snippet: Rabbit anti-TRPC1 antibody directed against an extracellular epitope of human TRPC1 (ACC-010), rabbit anti-TRPC3 antibody directed against an intracellular C-terminal epitope of mouse TRPC3 (ACC-016), rabbit anti-TRPC4 antibody directed against an intracellular C-terminal epitope of
Techniques: Functional Assay, Expressing, Activation Assay, Immunostaining